TIMP2 antibody (N-Term)

Catalog No. ABIN6940735

This anti-TIMP2 antibody is a Mouse Monoclonal antibody detecting TIMP2 in WB, IHC, IF, FACS and StM. Suitable for Human, Rat, Mouse, Cow, Rabbit, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for TIMP2 antibody (N-Term) (ABIN6940735)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: 3A4

Product Details anti-TIMP2 Antibody

Binding Specificity: AA 56-66, N-Term

Specificity: It recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Purification: Purified by Protein A/G

Immunogen: A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP2

Molecular Weight: 21kDa

Gene ID: 7077

UniProt: P16035

Pathways: cAMP Metabolic Process