PDLIM1 antibody

Catalog No. ABIN6941009

This anti-PDLIM1 antibody is a Mouse Monoclonal antibody detecting PDLIM1 in WB, IHC and StM. Suitable for Human.

Quick Overview for PDLIM1 antibody (ABIN6941009)

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDLIM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: CPTC-PDLIM1-1

Product Details anti-PDLIM1 Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length PDLIM1 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HepG2 cells and extracts from Jurkat. Human heart, colon or spleen.

Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PDLIM1

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Alternative Name: PDLIM1

Background: CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88 % sequence similarity with its rat counterpart, suggesting a conserved function between species.

Molecular Weight: 36kDa

Gene ID: 9124

UniProt: O00151