Recombinant CD8 alpha antibody

Catalog No. ABIN6941071

This Mouse Monoclonal antibody specifically detects CD8 alpha in IHC, ELISA, StM, Coat. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD8 alpha antibody (ABIN6941071)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Staining Methods (StM), Coating (Coat)

Clone: RC8-468

Product Details anti-CD8 alpha Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human CD8A protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HuT78 or hPBL. Tonsil.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8A

Background: CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

Molecular Weight: 32kDa

Gene ID: 925

UniProt: P01732

Pathways: TCR Signaling