CD8 alpha antibody (C-Term, Cytoplasmic Domain)

Catalog No. ABIN6941075

This Mouse Monoclonal antibody specifically detects CD8 alpha in FACS, IHC, IF, StM. It exhibits reactivity toward Human.

Quick Overview for CD8 alpha antibody (C-Term, Cytoplasmic Domain) (ABIN6941075)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Clone: C8-144B

Product Details anti-CD8 alpha Antibody

Binding Specificity: C-Term, Cytoplasmic Domain

Purification: Purified by Protein A/G

Immunogen: A 13 amino acid peptide from C-terminal cytoplasmic domain of alpha chain of human CD8 molecule.

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HuT78 or hPBL. Tonsil.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8A

Background: CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

Molecular Weight: 32kDa

Gene ID: 925

UniProt: P01732

Pathways: TCR Signaling