CD163 antibody (AA 43-196)

Catalog No. ABIN6941115

The Mouse Monoclonal anti-CD163 antibody has been validated for IHC, ELISA, ISt, Coat and StM. It is suitable to detect CD163 in samples from Human.

Quick Overview for CD163 antibody (AA 43-196) (ABIN6941115)

Target: CD163

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunostaining (ISt), Coating (Coat), Staining Methods (StM)

Clone: M130-2162

Product Details anti-CD163 Antibody

Binding Specificity: AA 43-196

Specificity: This MAb recognizes a protein of 140 kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 43-196) of human CD163 (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: HPBL cells. Skin, Lung, Placenta or Histiocytoma.

Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD163

Target: CD163

Alternative Name: CD163

Molecular Weight: 140kDa

Gene ID: 9332