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CD63 antibody (AA 100-197)

This anti-CD63 antibody is a Mouse Monoclonal antibody detecting CD63 in WB, IHC, ISt and StM. Suitable for Human and Mouse.
Catalog No. ABIN6941235

Quick Overview for CD63 antibody (AA 100-197) (ABIN6941235)

Target

See all CD63 Antibodies
CD63

Reactivity

  • 148
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  • 13
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Human, Mouse

Host

  • 83
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Mouse

Clonality

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Monoclonal

Conjugate

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This CD63 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Clone

LAMP3-2788
  • Binding Specificity

    • 22
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    AA 100-197

    Specificity

    This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

    Purification

    Purified by Protein A/G

    Immunogen

    Recombinant human CD63 protein fragment (around aa100-197) (exact sequence is proprietary)

    Isotype

    IgG2b kappa
  • Application Notes

    Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Human spleen or lymphoma tissue (IHC).

    Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions

    For Research Use only
  • Concentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Expiry Date

    24 months
  • Target

    CD63

    Alternative Name

    CD63

    Molecular Weight

    26kDa (core protein), 30-60kDa (glycosylated)

    Gene ID

    967

    UniProt

    P08962
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