Keratin 7/17 antibody

Catalog No. ABIN6941343

This Mouse Monoclonal antibody specifically detects Keratin 7/17 in IHC, WB, FACS, IF and StM. It exhibits reactivity toward Human and Cow.

Quick Overview for Keratin 7/17 antibody (ABIN6941343)

Target: Keratin 7/17 (KRT7/17)

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 7/17 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Clone: C-46

Product Details anti-Keratin 7/17 Antibody

Specificity: It recognizes intermediate filament proteins (IFP's) of 55 kDa and 46 kDa, which are identified as cytokeratin 7 and Cytokeratin 17 respectively. On formalin-fixed, paraffin embedded sections, C-46 reacts with keratin 7 only. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Cytokeratin 17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. Cytokeratin 17 antibody marks basal cell differentiation.

No Cross-Reactivity: Ferret, Mouse (Murine), Rabbit, Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Cytoskeletal preparation from HeLa cells (human cervical carcinoma cells)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa or ME-180 cells. Skin or hepatocellular carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Keratin 7/17

Target: Keratin 7/17 (KRT7/17)

Alternative Name: KRT7 & KRT17

Molecular Weight: 55kDa (CK7), 46kDa (CK17)

Gene ID: 3855, 3872

UniProt: P08729, Q04695