Recombinant Cytokeratin, Multi (Epithelial Marker) antibody
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- Target
- Cytokeratin, Multi (Epithelial Marker)
- Antibody Type
- Recombinant Antibody
- Reactivity
- Cow, Frog, Goat, Guinea Pig, Human, Monkey, Mouse, Pig, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Immunohistochemistry (IHC), Staining Methods (StM)
- Specificity
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
- Purification
- Purified by Protein A/G
- Immunogen
- Keratin-enriched preparation from cultured human epithelial cells
- Clone
- RKRT-457
- Isotype
- IgG1 kappa
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anti-Cytokeratin, Multi (Epithelial Marker) antibody
Reactivity: Cow, Frog, Goat, Guinea Pig, Human, Monkey, Mouse, Pig, Rat IHC, StM, FACS, IF, WB Host: Rabbit Monoclonal KRT-1877R unconjugated Recombinant Antibody
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- Application Notes
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Positive Control: A431 cells, Skin, Colon carcinoma.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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