ATP6V1G2 antibody (AA 51-118) (AbBy Fluor® 750)

Catalog No. ABIN6942187

The Rabbit Polyclonal anti-ATP6V1G2 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect ATP6V1G2 in samples from Mouse and Rat.

Quick Overview for ATP6V1G2 antibody (AA 51-118) (AbBy Fluor® 750) (ABIN6942187)

Target: ATP6V1G2 (ATPase, H+ Transporting, Lysosomal 13kDa, V1 Subunit G2 (ATP6V1G2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1G2 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ATP6V1G2 Antibody (AbBy Fluor® 750)

Binding Specificity: AA 51-118

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ATP6V1G2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ATP6V1G2

Target: ATP6V1G2 (ATPase, H+ Transporting, Lysosomal 13kDa, V1 Subunit G2 (ATP6V1G2))

Alternative Name: ATP6V1G2

Background:

Synonyms: ATP6G, ATP6G2, ATPase H+ transporting lysosomal (vacuolar proton pump) subunit G, ATPase H+ transporting lysosomal 13 kDa V1 subunit G2, H(+) transporting two sector ATPase subunit G2, NG 38, NG38, V ATPase 13 kDa subunit 2, V ATPase G subunit 2, V ATPase subunit G 2, Vacuolar ATP synthase subunit G 2, Vacuolar proton pump G subunit 2, Vacuolar proton pump subunit G 2, VMA 10, VMA10.

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of three V1 domain G subunit proteins. This gene had previous gene symbols of ATP6G and ATP6G2. Alternatively spliced transcript variants encoding different isoforms have been described. Read-through transcription also exists between this gene and the downstream DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B (DDX39B) gene. [provided by RefSeq, Feb 2011]

Gene ID: 534

UniProt: O95670

Pathways: Transition Metal Ion Homeostasis, Proton Transport