CD3G antibody

Catalog No. ABIN6942433

The Rabbit Monoclonal anti-CD3G antibody has been validated for WB, FACS and IHC (p). It is suitable to detect CD3G in samples from Human and Mouse.

Quick Overview for CD3G antibody (ABIN6942433)

Target: CD3G (CD3g Molecule, gamma (CD3-TCR Complex) (CD3G))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD3G antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2B11

Product Details anti-CD3G Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant protein within C-terminal human CD3G

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CD3G

Target: CD3G (CD3g Molecule, gamma (CD3-TCR Complex) (CD3G))

Alternative Name: CD3G

Background:

Synonyms: T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain, CD3g, CD3G, T3G

Background: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed:8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.

Gene ID: 917

UniProt: P09693

Pathways: TCR Signaling, CXCR4-mediated Signaling Events