IQSEC1 antibody (AA 451-550) (HRP)

Catalog No. ABIN6943527

This Rabbit Polyclonal antibody specifically detects IQSEC1 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for IQSEC1 antibody (AA 451-550) (HRP) (ABIN6943527)

Target: IQSEC1 (IQ Motif and Sec7 Domain 1 (IQSEC1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IQSEC1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IQSEC1 Antibody (HRP)

Binding Specificity: AA 451-550

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GEP100

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IQSEC1

Target: IQSEC1 (IQ Motif and Sec7 Domain 1 (IQSEC1))

Alternative Name: GEP100/IQSEC1

Background:

Synonyms: ADP-ribosylation factors guanine nucleotide-exchange protein 100, ADP-ribosylation factors guanine nucleotide-exchange protein 2, ARF GEP100, ARFGEP100, BRAG2, Brefeldin A resistant ARF GEF2, Brefeldin-resistant Arf-GEF 2 protein, GEP100, IQ motif and Sec7 domain 1, IQ motif and SEC7 domain-containing protein 1, IQEC1_HUMAN, Iqsec1, Kiaa0763.

Background: The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF6 plays a role in protein trafficking near the plasma membrane, including receptor recycling, cell adhesion and cell migration. ARF6 colocalizes with the ARF guanine nucleotide-exchange protein (GEP) BRAG2, also designated GEP100. BRAG2 is ubiquitously expressed as two isoforms, BRAG2a and BRAG2b, which can cycle between the cytoplasm and the nucleus. BRAG2, via its interaction with ARF6, is involved in the regulation of cell adhesion by controlling Integrin 1 endocytosis and E-cadherin redistribution. BRAG2 has also been shown to bind directly to Tyr1068/1086-phosphorylated EGFR to activate ARF6, which induces tumor invasion in MCF7 cells. Therefore, BRAG2 may contribute to the metastasis and malignancy of some breast cancer cells.

Gene ID: 9922

UniProt: Q6DN90