MYST1 antibody

Catalog No. ABIN6943861

The Rabbit Monoclonal anti-MYST1 antibody has been validated for WB, IF (cc), IF (p), IHC (p) and FACS. It is suitable to detect MYST1 in samples from Human, Mouse and Rat.

Quick Overview for MYST1 antibody (ABIN6943861)

Target: MYST1 (KAT8) (K(lysine) Acetyltransferase 8 (KAT8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MYST1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 2B1

Product Details anti-MYST1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein within N-terminal human KAT8.

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MYST1

Target: MYST1 (KAT8) (K(lysine) Acetyltransferase 8 (KAT8))

Alternative Name: KAT8

Background:

Synonyms: Histone acetyltransferase KAT8, Lysine acetyltransferase 8, MOZ YBF2/SAS3 SAS2 and TIP60 protein 1, MYST-1, hMOF, KAT8, MOF, MYST1

Background: Dosage compensation ensures that males with a single X chromosome and females with two X chromosomes have the same amount of most X-linked gene products. In Drosophila, this is acheived by enhancing the level of transcription of the X chromosome in males. Proteins such as maleless, male specific lethal 1, 2 and 3, and males absent on the first (MOF) form a dosage compensation complex (DCC) that is required for the twofold increase of transcription of the male X chromosome. The DCC is preferentially associated with many sites on the X chromosome in somatic cells of males. The binding of the DCC to the X chromosome is dependent upon histone 4 acetylation at lysine 16, which is accomplished by MOF. In mammals, MOF (also designated hMOF, MYST1, or MOZ) belongs to the MYST family of histone acetyl transferases which are characterized by a unique C2HC-type zinc finger close to their HAT domains. MOF utilizes the zinc finger domain to contact the globular part of the nucleosome as well as the histone H4 N-terminal tail substrate. The carboxy terminal domain of human MOF also has histone acetyltransferase activity directed against histones H3 and H2A, a characteristic shared with other MYST family histone acetyltransferases.

Gene ID: 84148

UniProt: Q9H7Z6