RhoA,B,C antibody

Catalog No. ABIN6944781

This Rabbit Monoclonal antibody specifically detects in WB, IHC, FACS, IF (cc) and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RhoA,B,C antibody (ABIN6944781)

Target: RhoA,B,C

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2G9

Product Details

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant human RHOC protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details

Target: RhoA,B,C

Alternative Name: Rho A+B+C

Background:

Synonyms: Transforming protein RhoA, Rho cDNA clone 12, Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, Rho-related GTP-binding protein RhoC, Rho cDNA clone 9, h12, h6, h9, RHOA, RHOB, RHOC, RHOH12, RHOH6, RHOH9

Background: The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50 % amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30 % homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine, A, aliphatic amino acid, X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

UniProt: P08134, P61586, P62745