SRSF3 antibody

Catalog No. ABIN6944869

The Rabbit Monoclonal anti-SRSF3 antibody is suitable to detect SRSF3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and IF (cc).

Quick Overview for SRSF3 antibody (ABIN6944869)

Target: SRSF3 (serine/arginine-Rich Splicing Factor 3 (SRSF3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SRSF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 9C11

Product Details anti-SRSF3 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Full length recombinant protein.

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SRSF3

Target: SRSF3 (serine/arginine-Rich Splicing Factor 3 (SRSF3))

Alternative Name: SFRS3

Background:

Synonyms: Serine/arginine-rich splicing factor 3, SRSF3, Pre-mRNA-splicing factor SRP20, Splicing factor arginine/serine-rich 3, SRP20

Background: Splicing factor that specifically promotes exon-inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. Also functions as export adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway), enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. RNA-binding is semi-sequence specific

Gene ID: 6428

UniProt: P84103