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Taq DNA Polymerase antibody

This anti-Taq DNA Polymerase antibody is a Mouse Monoclonal antibody detecting Taq DNA Polymerase in PCR. Suitable for Thermus aquaticus.
Catalog No. ABIN6952138

Quick Overview for Taq DNA Polymerase antibody (ABIN6952138)

Target

Taq DNA Polymerase

Reactivity

Thermus aquaticus

Host

  • 1
Mouse

Clonality

  • 1
Monoclonal

Conjugate

  • 1
This Taq DNA Polymerase antibody is un-conjugated

Application

  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Polymerase Chain Reaction (PCR)
  • Purpose

    The anti-Taq antibody inhibits the Taq DNA Polymerase at room temperature to increase specificity and sensitivity of the PCR.

    Specificity

    • Inhibits polymerase activity until desired
    • Enhances polymerase performance & stability under harsh cycling conditions
    • No DNA contaminations detected with PCR

    Characteristics

    HotStart Antibody is a highly pure antibody that binds to Taq DNA Polymerase and thereby blocks its polymerase activity. At the very beginning of the thermal cycling protocol, the antibody is inactivated and with this ""Hot Start"", Taq DNA Polymerase regains its function. This highly controlled reaction start prevents non-specific amplification and grants maximum control over the reaction conditions.

    Purification

    Affinity Column

    Immunogen

    Taq DNA Polymerase

    Isotype

    IgG
  • Application Notes

    Use 20 ng antibody for 1 Unit Taq DNA Polymerase

    Comment

    For convenience, 5 U/μl Taq polymerase can be mixed with an equal volume of HotStart antibody, aliquoted and stored at -20°C until further use.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    5 U/μL

    Buffer

    TE-Buffer, pH 7.4

    Storage

    -20 °C
  • Target

    Taq DNA Polymerase

    Alternative Name

    Taq Polymerase

    Molecular Weight

    94 kDa
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