CTCF antibody

Catalog No. ABIN6952397

This Rabbit Polyclonal antibody specifically detects CTCF in WB, ELISA, IF, ChIP and ChIP-seq. It exhibits reactivity toward Human.

Quick Overview for CTCF antibody (ABIN6952397)

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTCF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Grade: ChIP-seq Grade

Product Details anti-CTCF Antibody

Specificity: Polyclonal antibody raised in rabbit against human CTCF (CCCTC-Binding Factor), using 4 KLH coupled peptides.

Cross-Reactivity: Mouse (Murine)

Purification: Peptide affinity purified

Immunogen: synthetic peptide

Application Details

Application Notes: ChIP/ChIP-seq 1 μg:ChIP
ELISA 1:10,000
WB 1:1,000
IF 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.5 μg/μL

Buffer: PBS, 0.05 % azide, 0.05 % ProClin300

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Alternative Name: CTCF

Background: CTCF (UniProt/Swiss-Prot entry P49711) is a transcriptional regulator protein with 11 highly conserved zinc finger domains. By using different combinations of the zinc finger domains, CTCF can bind to different DNA sequences and proteins. As such it can act as both a transcriptional repressor and a transcriptional activator. By binding to transcriptional insulator elements, CTCF can also block communication between enhancers and upstream promoters, thereby regulating imprinted gene expression. CTCF also binds to the H19 imprinting control region and mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Mutations in the CTCF gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumor.

UniProt: P49711