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Recombinant SARS-CoV-2 Nucleocapsid antibody

The Human Monoclonal anti-SARS-CoV-2 Nucleocapsid antibody has been validated for ELISA and IF. It is suitable to detect SARS-CoV-2 Nucleocapsid in samples from SARS Coronavirus-2 (SARS-CoV-2) and SARS Coronavirus (SARS-CoV). There is 1 publication available.
Catalog No. ABIN6953058

Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid antibody (ABIN6953058)

Target

See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) Antibodies
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Antibody Type

Recombinant Antibody

Reactivity

  • 85
  • 7
  • 4
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV)

Host

  • 34
  • 30
  • 24
  • 4
  • 1
  • 1
Human

Clonality

  • 71
  • 16
  • 6
Monoclonal

Conjugate

  • 71
  • 15
  • 5
  • 1
  • 1
This SARS-CoV-2 Nucleocapsid antibody is un-conjugated

Application

  • 83
  • 39
  • 11
  • 10
  • 8
  • 6
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunofluorescence (IF)

Clone

CR3018 (03-018)
  • Purpose

    Anti-Covid-19 & SARS-CoV Nucleoprotein [CR3018 (03-018)], Human IgM, kappa

    Specificity

    This antibody binds the amino acid residues between 11-19 of the N protein of the SARS CoV as well as SARS-CoV-2 (COVID-19) nucleocapsid protein.

    Cross-Reactivity

    SARS Coronavirus-2 (SARS-CoV-2)

    Characteristics

    Original Species of Ab: Human

    Original Format of Ab: IgG1

    Purification

    Affinity Purified using a recombinant lectin column

    Immunogen

    The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The harvested supernatents were then purified on protein A columns. The original immunogen was the whole irradiated virion.

    Isotype

    IgM kappa
  • Application Notes

    This antibody is recommended for detection of SARS CoV2 protein N (nucleoprotein). This antibody binds both the nucleocapsid protein of the SARS-CoV and SARS CoV-2 (2019-nCoV). Initial characterization of the antibody for binding to 2019-nCoV was done using ELISA. This antibody shows potential to be used for development of diagnostic assays. Various isotype versions of the antibody namely human IgG1, IgG3, IgM, IgA and the less common IgG2 and IgG4 are available for the investigation of their role in response to SARS CoV2. Competitive ELISA of this antibody with CR3009 suggests that both these antibodies bind different epitopes of the N protein of SARS CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Immunofluorescence staining was used to demonstrate binding of CR3018 to SARS-CoV infected Vero cells. (PMID:15650189)

    Comment

    This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS with 0.02 % Proclin 300.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • van den Brink, Ter Meulen, Cox, Jongeneelen, Thijsse, Throsby, Marissen, Rood, Bakker, Gelderblom, Martina, Osterhaus, Preiser, Doerr, de Kruif, Goudsmit: "Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus." in: Journal of virology, Vol. 79, Issue 3, pp. 1635-44, (2005) (PubMed).

  • Target

    SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

    Alternative Name

    SARS-CoV-2 Nucleocapsid Protein

    Target Type

    Viral Protein

    Background

    NP, NC, Protein N, Nucleocapsid protein, SARS-CoV Protein N, SARS-CoV Nucleocapsid protein, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV

    UniProt

    P0DTC9, P59595
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