MHC II (I-A/I-E) antibody (APC)

Catalog No. ABIN7278019

The Rat Monoclonal anti-MHC II (I-A/I-E) antibody has been validated for FACS. It is suitable to detect MHC II (I-A/I-E) in samples from Mouse.

Quick Overview for MHC II (I-A/I-E) antibody (APC) (ABIN7278019)

Target: MHC II (I-A/I-E)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MHC II (I-A/I-E) antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: M5-114-15-2

Product Details anti-MHC II (I-A/I-E) Antibody (APC)

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG2b kappa

Application Details

Application Notes: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.

Comment:

0.2 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for MHC II (I-A/I-E)

Target: MHC II (I-A/I-E)

Alternative Name: MHC Class II (I-A/I-E)

Background: The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

Gene ID: 14961, 14969

UniProt: P14483