CD8 alpha antibody (PE)

Catalog No. ABIN7278279

This Mouse Monoclonal antibody specifically detects CD8 alpha in FACS. It exhibits reactivity toward Human.

Quick Overview for CD8 alpha antibody (PE) (ABIN7278279)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: OKT8

Product Details anti-CD8 alpha Antibody (PE)

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG2a

Application Details

Application Notes: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.

Comment:

5 uL (0.06 ug)/test Human CD8 (OKT8) PE

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background: The OKT8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen- bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracel- lular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. The OKT8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. If used together with alternative antibodies Anti-Human CD8a clone RPA-T8 or Anti-Human CD8a clone Hit8a, the OKT8 antibody will not block binding of RPA-T8 or Hit8a.

Gene ID: 925

UniProt: P01732

Pathways: TCR Signaling