CD3 antibody (PE-Cy7)

Catalog No. ABIN7278385

This anti-CD3 antibody is a Mouse Monoclonal antibody detecting CD3 in FACS. Suitable for Human.

Quick Overview for CD3 antibody (PE-Cy7) (ABIN7278385)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to PE-Cy7

Application: Flow Cytometry (FACS)

Clone: OKT3

Product Details anti-CD3 Antibody (PE-Cy7)

Cross-Reactivity: Chimpanzee

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.

Comment:

5 μL (0.5 μg)/test

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: The OKT3 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The OKT3 antibody is a widely used phenotypic marker for human T cells. In addition, as the CD3e subunit of the TCR complex contains intracellular signaling domains, binding of OKT3 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a CD3e-mitogenic antibody complex provides further insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the widely used human CD3 antibodies OKT3 and UCHT1 (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335). OKT3 has also been shown to be cross-reactive with Chimpanzee CD3 and

Gene ID: 915, 916, 917

UniProt: P20963, A8MVP6, P07766, P09693

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway