PD-L1 antibody (PerCP-Cy5.5)

Catalog No. ABIN7278491

The Mouse Monoclonal anti-PD-L1 antibody has been validated for FACS. It is suitable to detect PD-L1 in samples from Human.

Quick Overview for PD-L1 antibody (PerCP-Cy5.5) (ABIN7278491)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is conjugated to PerCP-Cy5.5

Application: Flow Cytometry (FACS)

Clone: 29E-2A3

Product Details anti-PD-L1 Antibody (PerCP-Cy5.5)

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG2b kappa

Application Details

Application Notes: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.

Comment:

5μl (1μg)/test

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide 0.1 % gelatin

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C protected from light

Expiry Date: 12 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1 (B7-H1)

Background: The 29E.2A3 antibody reacts with human CD274 which is also known as PD-L1 or B7-H1. CD274 is a type 1 transmembrane glycoprotein and a member of the Ig superfamily. PD-L1 is a ligand for CD279 (PD-1) and plays an important role in regulating immune cell responses. CD274 is expressed on activated monocytes, macrophages, dendritic cells, and T cells.

Gene ID: 29126

Pathways: Cancer Immune Checkpoints