MHC II (I-A/I-E) antibody

Catalog No. ABIN6961761

The Rat Monoclonal anti-MHC II (I-A/I-E) antibody has been validated for FACS, IP, IF, WB, IHC (fro) and IEM. It is suitable to detect MHC II (I-A/I-E) in samples from Mouse.

Quick Overview for MHC II (I-A/I-E) antibody (ABIN6961761)

Target: MHC II (I-A/I-E)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MHC II (I-A/I-E) antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)

Clone: M5-114-15-2

Product Details anti-MHC II (I-A/I-E) Antibody

Purification: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Isotype: IgG2b kappa

Application Details

Application Notes: This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Comment:

0.5 mg/mL

Restrictions: For Research Use only

Handling

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

Expiry Date: 12 months

Target Details for MHC II (I-A/I-E)

Target: MHC II (I-A/I-E)

Alternative Name: MHC Class II (I-A/I-E)

Background: The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

Gene ID: 14961, 14969

UniProt: P14483