Interferon gamma antibody (Biotin)

Catalog No. ABIN6963758

This Mouse Monoclonal antibody specifically detects Interferon gamma in ELISA and ELISpot. It exhibits reactivity toward Ferret and has been mentioned in 5+ publications.

Quick Overview for Interferon gamma antibody (Biotin) (ABIN6963758)

Target: Interferon gamma (IFNG)

Reactivity: Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: MTF19

Product Details anti-Interferon gamma Antibody (Biotin)

Specificity: Native and recombinant IFN-γ

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant ferret IFN-gamma

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-Interferon gamma antibody (Biotin) (ABIN6963758)

Gooch, Marriott, Ryan, Yeates, Slack, Brown, Fothergill, Whittaker, Carroll: "Heterosubtypic cross-protection correlates with cross-reactive interferon-gamma-secreting lymphocytes in the ferret model of influenza." in: Scientific reports, Vol. 9, Issue 1, pp. 2617, (2020) (PubMed).

Ryan, Slack, Marriott, Kane, Whittaker, Silman, Carroll, Gooch: "Cellular immune response to human influenza viruses differs between H1N1 and H3N2 subtypes in the ferret lung." in: PLoS ONE, Vol. 13, Issue 9, pp. e0202675, (2019) (PubMed).

Chan, Carolan, Korenkov, Druce, McCaw, Reading, Barr, Laurie: "Investigating Viral Interference Between Influenza A Virus and Human Respiratory Syncytial Virus in a Ferret Model of Infection." in: The Journal of infectious diseases, Vol. 218, Issue 3, pp. 406-417, (2019) (PubMed).

DiPiazza, Richards, Liu, Albrecht, Sant: "Analyses of Cellular Immune Responses in Ferrets Following Influenza Virus Infection." in: Methods in molecular biology (Clifton, N.J.), Vol. 1836, pp. 513-530, (2019) (PubMed).

Laurie, Horman, Carolan, Chan, Layton, Bean, Vijaykrishna, Reading, McCaw, Barr: "Evidence for Viral Interference and Cross-reactive Protective Immunity Between Influenza B Virus Lineages." in: The Journal of infectious diseases, Vol. 217, Issue 4, pp. 548-559, (2019) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Gene ID: 101693741

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy