APOE antibody (Biotin)

Catalog No. ABIN6963938

This Mouse Monoclonal antibody specifically detects APOE in WB, ELISA and ELISpot. It exhibits reactivity toward Human and Non-Human Primate and has been mentioned in 5+ publications.

Quick Overview for APOE antibody (Biotin) (ABIN6963938)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOE antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, ELISpot

Clone: E887

Product Details anti-APOE Antibody (Biotin)

Specificity: Native and recombinant apolipoprotein E (isoforms apoE2, apoE3 and apoE4).

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with apoE from non-human primates.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Purified human apolipoprotein E

Isotype: IgG1

Application Details

Application Notes: For quantification of apoE in serum/plasma samples and cell culture supernatants using ELISA and for enumeration of human apoE producing cells using ELISpot. E887 is recommended as detection mAb in combination with coating mAb E276 . The antibody is also suitable for immunoprecipitation and Western blot.

Comment:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-APOE antibody (Biotin) (ABIN6963938)

Bissig-Choisat, Wang, Legras, Saha, Chen, Bell, Pankowicz, Hill, Barzi, Leyton, Leung, Kruse, Himes, Goss, Wilson, Chan, Lagor, Bissig: "Development and rescue of human familial hypercholesterolaemia in a xenograft mouse model." in: Nature communications, Vol. 6, pp. 7339, (2015) (PubMed).

Bankwitz, Vieyres, Hueging, Bitzegeio, Doepke, Chhatwal, Haid, Catanese, Zeisel, Nicosia, Baumert, Kaderali, Pietschmann: "Role of hypervariable region 1 for the interplay of hepatitis C virus with entry factors and lipoproteins." in: Journal of virology, Vol. 88, Issue 21, pp. 12644-55, (2014) (PubMed).

Braesch-Andersen, Paulie, Smedman, Mia, Kumagai-Braesch: "ApoE production in human monocytes and its regulation by inflammatory cytokines." in: PLoS ONE, Vol. 8, Issue 11, pp. e79908, (2014) (PubMed).

Handattu, Nayyar, Garber, Palgunachari, Monroe, Keenum, Mishra, Datta, Anantharamaiah: "Two apolipoprotein E mimetic peptides with similar cholesterol reducing properties exhibit differential atheroprotective effects in LDL-R null mice." in: Atherosclerosis, Vol. 227, Issue 1, pp. 58-64, (2013) (PubMed).

Liu, Kuhel, Shen, Hui, Woods: "Apolipoprotein E does not cross the blood-cerebrospinal fluid barrier, as revealed by an improved technique for sampling CSF from mice." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 303, Issue 9, pp. R903-8, (2013) (PubMed).

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: apoE

Gene ID: 348

Pathways: Regulation of Cell Size, Lipid Metabolism