APOE antibody

Catalog No. ABIN6963954

The Rat Monoclonal anti-APOE antibody has been validated for ELISA. It is suitable to detect APOE in samples from Mouse. There are 2+ publications available.

Quick Overview for APOE antibody (ABIN6963954)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This APOE antibody is un-conjugated

Application: ELISA

Clone: ME1

Product Details anti-APOE Antibody

Specificity: Apolipoprotein E

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant mouse apoE

Isotype: IgG2a

Application Details

Application Notes: For quantification of mouse apoE in solution e.g. serum/plasma samples and cell culture supernatants using ELISA. mE1 is recommended as coating mAb in combination with biotinylated detection mAb mE29 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-APOE antibody (ABIN6963954)

Gordon, Yao, Xu, Karkowsky, Kaler, Kalchiem-Dekel, Barochia, Gao, Keeran, Jeffries, Levine: "Apolipoprotein E is a concentration-dependent pulmonary danger signal that activates the NLRP3 inflammasome and IL-1β secretion by bronchoalveolar fluid macrophages from asthmatic subjects." in: The Journal of allergy and clinical immunology, Vol. 144, Issue 2, pp. 426-441.e3, (2020) (PubMed).

Centa, Prokopec, Garimella, Habir, Hofste, Stark, Dahdah, Tibbitt, Polyzos, Gisterå, Johansson, Maeda, Hansson, Ketelhuth, Coquet, Binder, Karlsson, Malin: "Acute Loss of Apolipoprotein E Triggers an Autoimmune Response That Accelerates Atherosclerosis." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 38, Issue 8, pp. e145-e158, (2019) (PubMed).

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: apoE

Gene ID: 11816

Pathways: Regulation of Cell Size, Lipid Metabolism