APOE antibody (Biotin)

Catalog No. ABIN6963955

This anti-APOE antibody is a Rat Monoclonal antibody detecting APOE in WB and ELISA. Suitable for Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for APOE antibody (Biotin) (ABIN6963955)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This APOE antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: ME29

Product Details anti-APOE Antibody (Biotin)

Specificity: Apolipoprotein E

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant mouse apoE

Isotype: IgG2a

Application Details

Application Notes: For quantification of mouse apoE in serum/plasma samples and cell culture supernatants using ELISA. mE29 is recommended as detection mAb in combination with coating mAb mE1 . The antibody is also suitable for immunoprecipitation and Western blot.

Comment:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-APOE antibody (Biotin) (ABIN6963955)

Gordon, Yao, Xu, Karkowsky, Kaler, Kalchiem-Dekel, Barochia, Gao, Keeran, Jeffries, Levine: "Apolipoprotein E is a concentration-dependent pulmonary danger signal that activates the NLRP3 inflammasome and IL-1β secretion by bronchoalveolar fluid macrophages from asthmatic subjects." in: The Journal of allergy and clinical immunology, Vol. 144, Issue 2, pp. 426-441.e3, (2020) (PubMed).

Centa, Prokopec, Garimella, Habir, Hofste, Stark, Dahdah, Tibbitt, Polyzos, Gisterå, Johansson, Maeda, Hansson, Ketelhuth, Coquet, Binder, Karlsson, Malin: "Acute Loss of Apolipoprotein E Triggers an Autoimmune Response That Accelerates Atherosclerosis." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 38, Issue 8, pp. e145-e158, (2019) (PubMed).

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: apoE

Gene ID: 11816

Pathways: Regulation of Cell Size, Lipid Metabolism