IgE antibody

Catalog No. ABIN6963956

This Mouse Monoclonal antibody specifically detects IgE in ELISA and ELISpot. It exhibits reactivity toward Human and has been mentioned in 5+ publications.

Quick Overview for IgE antibody (ABIN6963956)

Target: IgE

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgE antibody is un-conjugated

Application: ELISA, ELISpot

Clone: 107

Product Details anti-IgE Antibody

Specificity: Native human IgE

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Human IgE

Isotype: IgG1

Application Details

Application Notes: For quantification of IgE in serum, plasma and cell culture supernatants using ELISA and for enumeration of IgE producing cells using ELISpot. 107 is recommended as coating mAb in combination with detection mAb 182 for ELISpot.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IgE antibody (ABIN6963956)

Asrat, Kaur, Liu, Ben, Kajimura, Murphy, Sleeman, Limnander, Orengo: "Chronic allergen exposure drives accumulation of long-lived IgE plasma cells in the bone marrow, giving rise to serological memory." in: Science immunology, Vol. 5, Issue 43, (2020) (PubMed).

Andersen, Mondal, Preissler, Freed, Stockinger, Bell, Druschel, Louis, Lawrence: "Detection of immunoglobulin isotypes from dried blood spots." in: Journal of immunological methods, Vol. 404, pp. 24-32, (2014) (PubMed).

Chowdhury, Chen, Yang, Cook, Nyborg, Ettinger, Herbst, Kiener, Wu: "Targeting the junction of CɛmX and ɛ-migis for the specific depletion of mIgE-expressing B cells." in: Molecular immunology, Vol. 52, Issue 3-4, pp. 279-88, (2012) (PubMed).

Wakamatsu, Satake, Igarashi, Dogru, Ibrahim, Okada, Fukagawa, Shimazaki, Fujishima: "IgE and eosinophil cationic protein (ECP) as markers of severity in the diagnosis of atopic keratoconjunctivitis." in: The British journal of ophthalmology, Vol. 96, Issue 4, pp. 581-6, (2012) (PubMed).

Scanlon, Hawksworth, Lane, Mahon: "IL-17A induces CCL28, supporting the chemotaxis of IgE-secreting B cells." in: International archives of allergy and immunology, Vol. 156, Issue 1, pp. 51-61, (2011) (PubMed).

Target Details for IgE

Target: IgE

Gene ID: 3497