IgE antibody (Biotin)

Catalog No. ABIN6963957

The Mouse Monoclonal anti-IgE antibody has been validated for ELISA. It is suitable to detect IgE in samples from Human. There are 5+ publications available.

Quick Overview for IgE antibody (Biotin) (ABIN6963957)

Target: IgE

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgE antibody is conjugated to Biotin

Application: ELISA

Clone: 182

Product Details anti-IgE Antibody (Biotin)

Specificity: Native human IgE

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Human IgE

Isotype: IgG2b

Application Details

Application Notes: For quantification of IgE in serum, plasma and cell culture supernatants using ELISA using ELISA. 182 is recommended as detection mAb in combination with coating mAb 107 .

Comment:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IgE antibody (Biotin) (ABIN6963957)

Asrat, Kaur, Liu, Ben, Kajimura, Murphy, Sleeman, Limnander, Orengo: "Chronic allergen exposure drives accumulation of long-lived IgE plasma cells in the bone marrow, giving rise to serological memory." in: Science immunology, Vol. 5, Issue 43, (2020) (PubMed).

Sánchez Ramírez, Morera Díaz, Bequet-Romero, Hernández-Bernal, Selman-Housein Bernal, de la Torre Santos, Santiesteban Álvarez, Martín Bauta, Bermúdez Badell, de la Torre Pupo, Gavilondo, Ayala Avila: "Characteristics of the specific humoral response in patients with advanced solid tumors after active immunotherapy with a VEGF vaccine, at different antigen doses and using two distinct adjuvants." in: BMC immunology, Vol. 18, Issue 1, pp. 39, (2018) (PubMed).

Bundhoo, Paveglio, Rafti, Dhongade, Blumberg, Matson: "Evidence that FcRn mediates the transplacental passage of maternal IgE in the form of IgG anti-IgE/IgE immune complexes." in: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Vol. 45, Issue 6, pp. 1085-98, (2016) (PubMed).

Andersen, Mondal, Preissler, Freed, Stockinger, Bell, Druschel, Louis, Lawrence: "Detection of immunoglobulin isotypes from dried blood spots." in: Journal of immunological methods, Vol. 404, pp. 24-32, (2014) (PubMed).

Wakamatsu, Satake, Igarashi, Dogru, Ibrahim, Okada, Fukagawa, Shimazaki, Fujishima: "IgE and eosinophil cationic protein (ECP) as markers of severity in the diagnosis of atopic keratoconjunctivitis." in: The British journal of ophthalmology, Vol. 96, Issue 4, pp. 581-6, (2012) (PubMed).

Target Details for IgE

Target: IgE

Gene ID: 3497