IL-31 antibody

Catalog No. ABIN6964030

This anti-IL-31 antibody is a Mouse Monoclonal antibody detecting IL-31 in Neut. Suitable for Human.

Quick Overview for IL-31 antibody (ABIN6964030)

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-31 antibody is un-conjugated

Application: Neutralization (Neut)

Clone: MT313

Product Details anti-IL-31 Antibody

Specificity: Native and recombinant human IL-31

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-31

Isotype: IgG1

Application Details

Application Notes: Neutralization of human IL-31 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: Neutralization of human IL-31 induced IL-6 production (see graph). To determine the neutralizing capacity and ND50, mixtures of human recombinant IL-31 (50 ng/ ml) and various concentrations of mAb MT313 were added to human aortic endothelial cells that had been pre-stimulated with recombinant human IFN-γ (10 ng/mL). Following overnight incubation, cell supernatants were quantified in IL-6 ELISA. Neutralization dose 50 (ND50): MT313 neutralized the effect of human IL-31 at a ND50value of 0.1-0.6 μg/mL when 50 ng/mL of human IL-31 was present. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS free of preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for IL-31

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Alternative Name: IL31

Gene ID: 386653