Histone H4 antibody (pHis18)

Catalog No. ABIN6972099

The Rabbit Polyclonal anti-Histone H4 antibody has been validated for WB and DB. It is suitable to detect Histone H4 in samples from Human.

Quick Overview for Histone H4 antibody (pHis18) (ABIN6972099)

Target: Histone H4

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Product Details anti-Histone H4 Antibody

Binding Specificity: pHis18

Characteristics: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Phosphorylation of histidines in Histone H4 has been known to occur in liver cells and may be associated with proliferation of hepatocytes during regeneration of the liver following damage. Histone H4H18ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a synthetic peptide containing a 3-phosphohistidine analog at Histidine 18 of human Histone H4.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG containing 30 % glycerol and 0.035 % sodium azide.
This serum was depleted over unmodified peptide before protein A purification.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Histone H4

Target: Histone H4

Molecular Weight: 8 kDa

NCBI Accession: NP_778224