Nanog antibody (AA 2-25)

Catalog No. ABIN6972401

This Rat Monoclonal antibody specifically detects Nanog in WB, IF and ICC. It exhibits reactivity toward Mouse.

Quick Overview for Nanog antibody (AA 2-25) (ABIN6972401)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 2E6E1

Product Details anti-Nanog Antibody

Binding Specificity: AA 2-25

Characteristics: Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity). Nanog antibody (mAb) (Clone 2E6E1) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Mouse samples.

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a peptide comprising amino acids 2-25 of mouse Nanog.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Nanog

Molecular Weight: 45 kDa

NCBI Accession: NP_001276757

Pathways: Stem Cell Maintenance