RNA Pol II CTD Ser2ph / Ser5ph (pSer2), (pSer5) antibody

Catalog No. ABIN6972651

The Mouse Monoclonal anti- antibody has been validated for ICC, IF, WB, ChIP-seq and ChIP. It is suitable to detect in samples from Human.

Quick Overview for RNA Pol II CTD Ser2ph / Ser5ph (pSer2), (pSer5) antibody (ABIN6972651)

Target: RNA Pol II CTD Ser2ph / Ser5ph

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Clone: 1A12G10

Product Details

Binding Specificity: pSer2, pSer5

Characteristics: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 2 phosphorylation is enriched over the gene body and is associated with transcriptional elongation. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription. RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) (Clone 1A12G10) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a synthetic peptide containing phospho-serine 2 and phospho-serine 5 of the CTD of human RNA Polymerase II.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details

Target: RNA Pol II CTD Ser2ph / Ser5ph

Molecular Weight: 240 kDa

NCBI Accession: NP_000928