Ubiquitin antibody (HRP)

Catalog No. ABIN6988053

This anti-Ubiquitin antibody is a Mouse Monoclonal antibody detecting Ubiquitin in IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Ubiquitin antibody (HRP) (ABIN6988053)

Target: Ubiquitin (Ub)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is conjugated to HRP

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 8A10

Product Details anti-Ubiquitin Antibody (HRP)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Ubiquitin

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background:

Synonyms: FLJ25987, MGC8385, RPS27A, RPS 27A, UBA52, UBA 52, UBA80, UBA 80, UBB, UBC, UB, UBCEP1, UBCEP2, UBCEP 1, UBCEP 2, UBCEP-1, UBCEP-2, Ubiquitin, ubiquitin B, UBC_HUMAN, UBB_HUMAN, RS27A_HUMAN, HMG20, Ubiquitin carboxyl extension protein 80, Ubiquitin, Polyubiquitin-C, Polyubiquitin-B.

Background: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Function : Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Gene ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway