RNF111 antibody (AA 851-950)

Catalog No. ABIN6989155

This Rabbit Polyclonal antibody specifically detects RNF111 in ELISA, IF (cc), IF (p), IHC (p), ICC and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for RNF111 antibody (AA 851-950) (ABIN6989155)

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF111 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RNF111 Antibody

Binding Specificity: AA 851-950

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RNF111

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RNF111

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Alternative Name: RNF111

Background:

Synonyms: ARK, Arkadia, DKFZp313E0731, DKFZp686H1966, DKFZp761D081, E3 ubiquitin protein ligase Arkadia, FLJ38008, Ring finger protein 111, RNF 111, RN111_HUMAN.

Background: The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

Gene ID: 54778

UniProt: Q6ZNA4