TOP2B antibody (AA 1-100)

Catalog No. ABIN6989203

This Rabbit Polyclonal antibody specifically detects TOP2B in ELISA, IHC (p), ICC, IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human and Rat.

Quick Overview for TOP2B antibody (AA 1-100) (ABIN6989203)

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP2B antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TOP2B Antibody

Binding Specificity: AA 1-100

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit,Chimpanzee

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Topoisomerase II beta

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for TOP2B

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Alternative Name: Topoisomerase II beta

Background:

Synonyms: Antigen MLAA 44, beta isozyme, DNA topoisomerase 2 beta, DNA topoisomerase 2-beta, DNA topoisomerase II 180 kD, DNA topoisomerase II, DNA topoisomerase II beta, DNA topoisomerase II beta isozyme, Top 2, TOP 2B, TOP IIB, Top2 beta, TOP2B, TOP2B_HUMAN, Top2beta, TOPIIB, Topo II beta, Topoisomerase (DNA) II beta 180 kDa, Topoisomerase (DNA) II beta, Topoisomerase IIb, U937 associated antigen.

Background: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Gene ID: 7155

UniProt: Q02880