XBP1 antibody (N-Term)

Catalog No. ABIN6990120

This anti-XBP1 antibody is a Rabbit Polyclonal antibody detecting XBP1 in WB, ELISA, IF, ICC and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for XBP1 antibody (N-Term) (ABIN6990120)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-XBP1 Antibody

Binding Specificity: AA 40-90, N-Term

Purification: XBP-1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-XBP-1 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human XBP-1. The immunogen is located within amino acids 40 - 90 of XBP-1.

Isotype: IgG

Application Details

Application Notes: WB: 0.25-2 μ,g/mL, IF: 20 μ,g/mL, ICC: 10 μ,g/mL, IHC: 2-5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunofluorescence in human and rat samples, Immunocytochemistry in human samples, Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: XBP-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: XBP-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP-1

Background: XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

Molecular Weight: Predicted: 28/40kD

Observed: 28/40 kD kDa

Gene ID: 7494

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response