BIRC7 antibody (C-Term)

Catalog No. ABIN6990386

The Rabbit Polyclonal anti-BIRC7 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect BIRC7 in samples from Human.

Quick Overview for BIRC7 antibody (C-Term) (ABIN6990386)

Target: BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIRC7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-BIRC7 Antibody

Binding Specificity: C-Term

Specificity: A lower but much weaker band at 30 kDa was detected in Raji cell lysate, which may represent the short form of Livin.

Purification: Livin Antibody is affinity chromatography purified via peptide column.

Immunogen: Livin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Livin. The immunogen is located within the last 50 amino acids of Livin.

Isotype: IgG

Application Details

Application Notes: Livin antibody can be used for detection of Livin by Western blot at 0.5 μ,g/mL. A band at 33 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Livin Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Livin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for BIRC7

Target: BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))

Alternative Name: Livin

Background: Livin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP. Livin/XIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-alpha and Livin-beta. Transfection of Livin in cells resulted in protection from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6. Livin has direct interaction with several caspases including caspase-3, -7, and -9. Livin inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. The two isoforms of Livin appear to have different functions and tissue distributions.

Molecular Weight: 33 kDa

Gene ID: 79444

NCBI Accession: NP_071444

UniProt: Q96CA5