CXCR4 antibody (AA 170-220)

Catalog No. ABIN6990403

This Rabbit Polyclonal antibody specifically detects CXCR4 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CXCR4 antibody (AA 170-220) (ABIN6990403)

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CXCR4 Antibody

Binding Specificity: AA 170-220

Purification: CXCR4 Antibody is Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-CXCR4 antibody was raised against a peptide corresponding to 15 amino acids near the center of human CXCR4 isoform b. The immunogen is located within amino acids 170-220 of CXCR4.

Isotype: IgG

Application Details

Application Notes: WB: 1 - 2 μ,g/mL, IHC-P: 5 μ,g/mL, IF: 4 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CXCR4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CXCR4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Alternative Name: CXCR4

Background:

CXCR4, a G-protein coupled receptor (GPCR) with seven transmembrane domains, is a CXC chemokine receptor specific for stromal-derived-factor-1 (SDF-1 or CXCL12). CXCR4 was initially discovered as one of the co-receptors for HIV entry into CD4+ T cells (1). Blocking CXCR4 could be potentially used as novel therapeutics for HIV treatment.

CXCR4 signaling plays an important role in the migration, proliferation and quiescence of hematopoietic stem cell and their retention within the bone marrow, where it has high levels of SDF-1/CXCL12(2). It has been demonstrated that CXCR4 signaling mediates CD20 up-regulation on B cells (3).

CXCR4 is highly expressed in more than 23 types of cancer, including breast cancer, ovarian cancer, melanoma, and prostate cancer, while there is very less or no expression of CXCR4 in healthy tissues. CXCR4 expression in cancer cells has been reported to be associated with tumor survival, growth and metastasis in tissues with high levels of SDF-1/CXCL12, such as lungs, liver and bone marrow (4,5).

CXCR4 has been shown to regulate neuronal migration, cell positioning and axon wiring (6,7). CXCR4 mutant mice displayed aberrant neuronal distribution, which implicates the role in neuronal disorders such as epilepsy. CXCR4 is also involved in WHIM syndrome (8). WHIM mutations in CXCR4 were recently found in patients with Waldenstrom's macroglobulinemia, and these mutations are correlated to clinical resistance to ibrutinib (9,10).

Molecular Weight: Predicted: 40kD

Observed: 44kD (Post-modification: 2 N-linked glycosylation) kDa

Gene ID: 7852

NCBI Accession: NP_003458

UniProt: P61073

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events