PHAP I antibody (C-Term)

Catalog No. ABIN6990424

This Rabbit Polyclonal antibody specifically detects PHAP I in WB, ELISA, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PHAP I antibody (C-Term) (ABIN6990424)

Target: PHAP I

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHAP I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PHAP I Antibody

Binding Specificity: C-Term

Specificity: This polyclonal antibody has no cross-reaction to PHAP I2a and PHAP III.

Purification: PHAP I Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-PHAP I antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human PHAP I. The immunogen is located within the last 50 amino acids of PHAP I.

Isotype: IgG

Application Details

Application Notes: WB: 2-4 μ,g/mL, ICC: 2 μ,g/mL, IF: 10 ?g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PHAP I Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PHAP I antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PHAP I

Target: PHAP I

Background: PHAP I Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

Molecular Weight: Predicted: 29kD

Observed: 29 kD kDa

Gene ID: 8125

UniProt: P39687