ATG7 antibody (C-Term)

Catalog No. ABIN6990575

The Rabbit Polyclonal anti-ATG7 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect ATG7 in samples from Human and Mouse.

Quick Overview for ATG7 antibody (C-Term) (ABIN6990575)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ATG7 Antibody

Binding Specificity: AA 590-640, C-Term

Specificity: At least three isoforms of APG7 are known to exist, this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.

Purification: APG7 Antibody is affinity chromatography purified via peptide column.

Immunogen: APG7 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7. The immunogen is located within amino acids 590 - 640 of APG7.

Isotype: IgG

Application Details

Application Notes: APG7 antibody can be used for the detection of APG7 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: APG7 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: APG7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: APG7

Background: APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme, this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

Molecular Weight: Predicted: 69, 74, 77 kDa

Observed: 77 kDa

Gene ID: 10533

NCBI Accession: NP_006386

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy