beta Actin antibody (N-Term)

Catalog No. ABIN6990626

The Rabbit Polyclonal anti-beta Actin antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect beta Actin in samples from Human, Mouse and Rat.

Quick Overview for beta Actin antibody (N-Term) (ABIN6990626)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Purification: beta-Actin Antibody is affinity chromatography purified via peptide column.

Immunogen: b-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human b-actin.

Isotype: IgG

Application Details

Application Notes: b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 1 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: beta-Actin Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta-Actin

Background: Beta-Actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Gene ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction