BRE antibody (N-Term)

Catalog No. ABIN6990794

The Rabbit Polyclonal anti-BRE antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect BRE in samples from Human, Mouse and Rat.

Quick Overview for BRE antibody (N-Term) (ABIN6990794)

Target: BRE (Brain and Reproductive Organ-Expressed (TNFRSF1A Modulator) (BRE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BRE Antibody

Binding Specificity: N-Term

Purification: BRCC45 Antibody is affinity chromatography purified via peptide column.

Immunogen: BRCC45 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC45. The immunogen is located within the last 50 amino acids of BRCC45.

Isotype: IgG

Application Details

Application Notes: BRCC45 antibody can be used for detection of BRCC45 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: BRCC45 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: BRCC45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for BRE

Target: BRE (Brain and Reproductive Organ-Expressed (TNFRSF1A Modulator) (BRE))

Alternative Name: BRCC45

Background: BRCC45 Antibody: BRCC45 was initially suggested to be a housekeeping protein that is highly expressed in brain and reproductive organs. Later experiments indicated BRCC45 forms a complex with the breast and ovarian predisposition proteins BRCA1 and BRCA2 as well as RAD51 and BRCC36. This complex has a ubiquitin E3 ligase activity and is thought to enhance cellular survival following DNA damage. BRCC45 has also been suggested to function as a death receptor-associated anti-apoptotic protein by inhibiting the BID-induced activation of the mitochondrial apoptotic pathway. Higher levels of BRCC45 were detected in the majority of hepatocellular carcinomas, suggesting that BRCC45 may promote tumorigenesis when overexpressed. At least three isoforms of BRCC45 are known to exist.

Gene ID: 9577

NCBI Accession: NP_004890

UniProt: Q9NXR7

Pathways: Positive Regulation of Response to DNA Damage Stimulus