IRGM antibody (N-Term)

Catalog No. ABIN6990882

This anti-IRGM antibody is a Rabbit Polyclonal antibody detecting IRGM in WB, ELISA, IHC (p) and IF. Suitable for Human, Mouse and Rat.

Quick Overview for IRGM antibody (N-Term) (ABIN6990882)

Target: IRGM (Immunity-Related GTPase Family, M (IRGM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRGM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-IRGM Antibody

Binding Specificity: AA 30-80, N-Term

Purification: IRGM Antibody is affinity chromatography purified via peptide column.

Immunogen: IRGM antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human IRGM. The immunogen is located within amino acids 30 - 80 of IRGM.

Isotype: IgG

Application Details

Application Notes: IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IRGM Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IRGM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IRGM

Target: IRGM (Immunity-Related GTPase Family, M (IRGM))

Alternative Name: IRGM

Background: IRGM Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn's disease. IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa that also includes IRGM2 and IRGM3, this antibody may also recognize these proteins.

Molecular Weight: Predicted: 20 kDa

Observed: 20 kDa 

Gene ID: 345611

UniProt: A1A4Y4