EBI3 antibody (AA 150-200)

Catalog No. ABIN6991042

This Rabbit Polyclonal antibody specifically detects EBI3 in WB, ELISA, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EBI3 antibody (AA 150-200) (ABIN6991042)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EBI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-EBI3 Antibody

Binding Specificity: AA 150-200

Purification: EBI3 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-EBI-3 antibody was raised against a peptide corresponding to 16 amino acids near the center of mature human EBI-3. The immunogen is located within amino acids 150 - 200 of EBI3.

Isotype: IgG

Application Details

Application Notes: WB: 0.5 - 2 μ,g/mL, IF: 20 μ,g/mL , IHC: 2.5 μ,g/mL,.

Antibody validated: Western Blot in human and rat samples, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: EBI3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: EBI3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for EBI3

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: EBI3

Background: EBI3 Antibody: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naive but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

Molecular Weight: Predicted: 25kD

Observed: 30 kD (25kD + 2 N-Glycosylations) kDa

Gene ID: 10148

NCBI Accession: NP_005746

UniProt: Q14213