N4BP1 antibody (N-Term)

Catalog No. ABIN6991485

This anti-N4BP1 antibody is a Rabbit Polyclonal antibody detecting N4BP1 in WB, ELISA, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for N4BP1 antibody (N-Term) (ABIN6991485)

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N4BP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-N4BP1 Antibody

Binding Specificity: N-Term

Specificity: At least three isoforms of N4BP1 are known to exist, this antibody will only detect the longest isoform.

Purification: N4BP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1. The immunogen is located within the first 50 amino acids of N4BP1.

Isotype: IgG

Application Details

Application Notes: N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: N4BP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: N4BP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for N4BP1

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Alternative Name: N4BP1

Background: N4BP1 Antibody: Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

Gene ID: 9683

NCBI Accession: NP_694574

UniProt: O75113