SIK1 antibody (AA 340-390)

Catalog No. ABIN6991527

This anti-SIK1 antibody is a Rabbit Polyclonal antibody detecting SIK1 in WB, ELISA and IHC (p). Suitable for Human.

Quick Overview for SIK1 antibody (AA 340-390) (ABIN6991527)

Target: SIK1 (Salt-Inducible Kinase 1 (SIK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIK1 Antibody

Binding Specificity: AA 340-390

Specificity: Two isoforms of SIK1 are known to exist, this antibody will detect both isoforms. SIK1 antibody is predicted to not cross-react with other SIK protein family members.

Purification: SIK1 Antibody is affinity chromatography purified via peptide column.

Immunogen: SIK1 antibody was raised against an 18 amino acid synthetic peptide near the center of human SIK1. The immunogen is located within amino acids 340 - 390 of SIK1.

Isotype: IgG

Application Details

Application Notes: SIK1 antibody can be used for detection of SIK1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SIK1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SIK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SIK1

Target: SIK1 (Salt-Inducible Kinase 1 (SIK1))

Alternative Name: SIK1

Background: SIK1 Antibody: Salt-inducible kinase 1 (SIK1), also known as SNF1LK or MSK, plays a role in histone modification and G2/M cell cycle regulation. It is a 783 amino acid protein that contains one UBA domain and belongs to the Ser/Thr protein kinase family (AMPK subfamily). Localized to both the nucleus and the cytoplasm, SIK1 is a class II HDAC kinase that uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation.

Gene ID: 150094

NCBI Accession: NP_775490

UniProt: P57059

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process