IRF4 antibody (C-Term)

Catalog No. ABIN6991531

This Rabbit Polyclonal antibody specifically detects IRF4 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IRF4 antibody (C-Term) (ABIN6991531)

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-IRF4 Antibody

Binding Specificity: C-Term

Specificity: IRF4 antibody is predicted to not cross-react with other IRF protein family members. IRF4 often migrates at a higher than predicted molecular weight in SDS-PAGE.

Purification: IRF4 Antibody is affinity chromatography purified via peptide column.

Immunogen: IRF4 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human IRF4. The immunogen is located within the last 50 amino acids of IRF4.

Isotype: IgG

Application Details

Application Notes: IRF4 antibody can be used for detection of IRF4 by Western blot at 0.5 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IRF4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: IRF4 antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IRF4

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Alternative Name: IRF4

Background: IRF4 Antibody: Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Another member, IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF4 expression is tightly regulated in resting primary T cells and plays an essential role in the homeostasis and function of mature lymphocytes. IRF4 is induced by Toll-like receptor (TLR) activation and acts as a negative regulator of TLR signaling.

Gene ID: 3662

NCBI Accession: NP_002451

UniProt: Q15306

Pathways: Chromatin Binding