VASH1 antibody (C-Term)

Catalog No. ABIN6991536

This anti-VASH1 antibody is a Rabbit Polyclonal antibody detecting VASH1 in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for VASH1 antibody (C-Term) (ABIN6991536)

Target: VASH1 (Vasohibin 1 (VASH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VASH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-VASH1 Antibody

Binding Specificity: AA 290-340, C-Term

Specificity: VASH1 antibody is predicted to not cross-react with other vasohibin protein family members. At least two isoforms are known to exist, this antibody will recognize only the long isoform.

Purification: VASH1 Antibody is affinity chromatography purified via peptide column.

Immunogen: VASH1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human VASH1. The immunogen is located within amino acids 290 - 340 of VASH1.

Isotype: IgG

Application Details

Application Notes: VASH1 antibody can be used for detection of VASH1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: VASH1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: VASH1 antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for VASH1

Target: VASH1 (Vasohibin 1 (VASH1))

Alternative Name: VASH1

Background: VASH1 Antibody: VASH1 was originally identified as an endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor that acts in a negative feedback manner. VASH1 mRNA is expressed brain, heart, kidney and placenta in the adult, with robust expression in various organs during embryonic development. VASH1 expression in tumor cells and tumor vasculature is silenced by methylation by increased expression of Zeste homolog 2 (EZH2). The increase of EZH2 expression is induced by VEGF stimulation, leading to poor clinical prognosis. Recombinant adenovirus expressing VASH prevented tumor angiogenesis and inhibited tumor growth, suggesting that it may be a potentially valuable antitumor therapy in the clinic.

Gene ID: 22846

NCBI Accession: NP_055724

UniProt: Q7L8A9