PTCHD2 antibody (AA 780-830)

Catalog No. ABIN6991628

The Rabbit Polyclonal anti-PTCHD2 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect PTCHD2 in samples from Human, Mouse and Rat.

Quick Overview for PTCHD2 antibody (AA 780-830) (ABIN6991628)

Target: PTCHD2 (Patched Domain Containing 2 (PTCHD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTCHD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-PTCHD2 Antibody

Binding Specificity: AA 780-830

Specificity: At least two isoforms of PTCHD2 are known to exist. This antibody is specific for PTCHD2 and will not recognize the other DISP family of proteins.

Purification: PTCHD2 Antibody is affinity chromatography purified via peptide column.

Immunogen: PTCHD2 antibody was raised against a 19 amino acid synthetic peptide from near the center of human PTCHD2. The immunogen is located within amino acids 780 - 830 of PTCHD2.

Isotype: IgG

Application Details

Application Notes: PTCHD2 antibody can be used for detection of EPAC1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PTCHD2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PTCHD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PTCHD2

Target: PTCHD2 (Patched Domain Containing 2 (PTCHD2))

Alternative Name: PTCHD2

Background: PTCHD2 Antibody: PTCHD2, also known as DISP3, is the third of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored secreted proteins. Like DISP1 and DISP2, DISP3 has been implicated in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis. It is highly expressed in Purkinje cells, hippocampal neurons, and retinal ganglion cells. Recently, it has been shown that PTCHD2 localizes within the endoplasmic reticulum and colocalizes with cholesterol, and given that its expression is regulated by thyroid hormone (T3), it has been suggested that DISP3 may be a link between thyroid hormone and cholesterol metabolism.

Gene ID: 57540

UniProt: Q9P2K9