LEMD2 antibody (AA 220-270)

Catalog No. ABIN6991701

Product Details anti-LEMD2 Antibody

Target: LEMD2 (LEM Domain Containing 2 (LEMD2))

Binding Specificity: AA 220-270

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEMD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: At least two isoforms of LEMD2 are known to exist, this antibody will detect only the longer isoform.

Purification: LEMD2 Antibody is affinity chromatography purified via peptide column.

Immunogen: LEMD2 antibody was raised against a 16 amino acid synthetic peptide near the center of human LEMD2. The immunogen is located within amino acids 220 - 270 of LEMD2.

Isotype: IgG

Application Details

Application Notes: LEMD2 antibody can be used for detection of LEMD2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LEMD2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LEMD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LEMD2

Target: LEMD2 (LEM Domain Containing 2 (LEMD2))

Alternative Name: LEMD2 (LEMD2 Products)

Background: LEMD2 Antibody: The LEM (lamina-associated polypeptide-emerin-MAN1) domain is a motif shared by a group of lamin-interacting proteins of the inner nuclear membrane (INM) and nucleoplasm. One such protein, LEMD2 (LEM domain-containing protein 2), is a lamina-associated protein in the INM involved in nuclear structure organization, containing an N-terminal LEM motif, two transmembrane domains, and a MAN1-Src1p C-terminal (MSC) domain. LEMD2 is a truncated paralog of MAN1, lacking the MAN1-specific C-terminal RNA-recognition motif, but like MAN1 is required for myogenic differentiation. Mutations in LEMD2 are thought be among the possible causes of muscle disease.

Gene ID: 221496

NCBI Accession: NP_851853

UniProt: Q8NC56